Practical protocol for monitoring biochemical markers during the sports season.

Practical Protocol for Monitoring Biochemical Markers During the Season

1. General Preparation Phase

  • Objective: To develop the general physical base.
  • Recommended Measures:
    • Creatine Kinase (CK): Once a week, 24 to 48 hours after intense strength and conditioning sessions.
    • Cortisol/Testosterone Ratio: Once every two weeks to actively monitor hormonal balance and anabolic/catabolic state.
    • Ferritin: Once at the beginning of the preparation phase to evaluate baseline iron reserves.

2. Specific Preparation Phase

  • Objective: To develop sport-specific qualities (speed, explosive power, anaerobic capacities, etc.).
  • Recommended Measures:
    • Blood Lactate: Analyzed during performance tests (speed or endurance testing) every two weeks.
    • Creatine Kinase (CK): Twice a week during periods of increased training load and volume.
    • Cortisol/Testosterone Ratio: Once a week.
    • CRP (C-Reactive Protein): Monitored solely in cases of abnormal systemic fatigue or suspected inflammation.

3. Competition Phase

  • Objective: To maintain peak performance levels and mitigate excessive neuromuscular and systemic fatigue.
  • Recommended Measures:
    • Creatine Kinase (CK): 24 hours after each official match or highly intense physical event.
    • Cortisol/Testosterone Ratio: Once every two weeks.
    • Blood Glucose: Tested on the morning of major competitions to verify the athlete's energetic and glycogen status.
    • CRP (C-Reactive Protein): In the event of persistent muscle soreness or clinical signs of fatigue.

4. Transition Phase (End of Season)

  • Objective: To facilitate complete recovery and physiological regeneration.
  • Recommended Measures:
    • Creatine Kinase (CK): Once, to verify full muscular recovery and tissue repair.
    • Ferritin: Re-assessment of iron stores post-season.
    • Cortisol/Testosterone Ratio: To evaluate and ensure the return to physiological hormonal homeostasis.
    • Blood Urea: To confirm the absence of excessive protein breakdown and muscle catabolism.

⚠️ Important Remarks

  • Sampling Conditions: Blood samples must be taken in the morning on an empty stomach (typically between 7:00 AM and 9:00 AM), strictly prior to any physical activity.
  • Individualized Interpretation: Data analysis must be highly individualized. Results should be compared against the athlete's own baseline values (historical data) rather than solely relying on general clinical norms.
  • Data Trends: The evolution and trending of the measurements over time are significantly more relevant than isolated absolute values. (Example: A +30% spike in CK levels compared to the athlete's usual baseline is a direct clinical sign of physical overload).